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The samples were analyzed with HPLC on a Waters 2690 separations module, using an AccQTag column (Waters) and fluorescence detection (Waters detector).
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Amino acid analysis was performed using a Waters AccQTag column.
RNA was then purified using an RNeasy spin column (Qiagen) and an on-column DNase treatment.
Pol IV was purified using a Capto S column, Hitrap Phenyl column, and Superdex 200 column.
We extracted DNA using an ion-exchange column method [ 34].
For the US, Yahoo is starting with a limited set of topics and using a two column instead of a three column design.
2-MEA was then removed using a desalting column.
Labeled antibodies were purified using a PD10 column (GE Healthcare).
(A ) Chromatogram of HPLC purification using a C18 column.
Then, excess biotin reagent was removed using a desalting column.
The samples were analyzed by GC using a Supelco column.
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