The number of neurons was estimated by using a 40x lens (final magnification, 1,450x), and the number of Purkinje cells, by using a 20x lens (final magnification, 720x).
Confocal microscopy was performed on a Zeiss (Thornwood, NY) LSM510 Meta microscope using a 40x lens.
The slides were observed with a Vanox-S Olympus light microscope (Tokyo, Japan) using a 40x lens.
Sections were observed with a laser scanning confocal microscope, TCS SP5 (Leica Microsystems, Mannheim) using a 40x (NA=1.25) lens with optical pinhole at 1AU.
I shot the eclipse from my front porch using a 600mm lens on a tripod, and took maybe 50 or 60 pictures.
The great French photographer Henri Cartier-Bresson was known for only using a 50mm lens on his Leica, and it doesn't seem to have done him any harm.
Using a 500mm lens, he peeked into the lives of others – like a real-life LB Jeffries from the film Rear Window – and obliterated the assumed divide between the public and the personal.
"I was using a 50mm lens—I was so close to them," Stones tells Creators.
The overall morphology of the filled cells was assessed by using a 20× lens, 0.5 NA.
For measurement of fluorescence intensity, images were taken using a 2x lens from ventral VNOs.
