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Images were reviewed and analyzed independently using Advantage Workstation (GE Healthcare).
Blood glucose concentration was measured (mmol/L) prior to FDG injection with a small drop of blood from the saphenous vein using Advantage blood glucose strips (AccuChek, Roche Diagnostics, Laval, QC, Canada).
SERS spectra were recorded using Advantage 532 and Advantage 200A Raman spectrometers (DeltaNu, Laramie, WY, USA) equipped with a double frequency NdYAG laser emitting at 532 nm (5-mW laser power) and a HeNe laser emitting at 632.8 nm (4-mW laser power), respectively.
The second strand was amplified using Advantage polymerase by 5′ PCR primer II A, 5′-A AGCAGTGGTATCAACGCAGAGT-3′. A directional cDNA library was constructed with a plasmid cloning kit (SuperScript™ Plasmid System, GIBCO/BRL) following the instructions of manufacturer, producing a library of about 1.0 × 106 independent colonies.
All PCR was performed using Advantage 2 Polymerase Mix (Clontech, Mountain View, CA).
The second strand was amplified using Advantage polymerase by 5' PCR primer II A, 5'-AAGCAGTGGTATCAACGCAGAGT 3'.
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