Sentence examples for using additional primer sets from inspiring English sources

Similar procedures were used to obtain partial cDNA sequences from the other equid species, using additional primer sets designed to overlap those of the original primer positions to ensure that the final sequences were fully correct for each species.

qRT-PCR using additional primer sets was done to validate five of these genes, which confirmed that mRNA expression levels of type I collagen, fibronectin, MMP-2, MMP-3, and MMP-9 were elevated in the stromal cells from Cdc42-overexpressing mammary glands.

Complementary PCR products were amplified using the additional primer sets MGR2/JSR01 and MGF1/JSR05.

These reductions in expression were also observed using three additional primer sets at downstream locations in the MALAT1 gene (Fig.  6f, g and Additional file 5: Figure S5).

To verify testis-specificity of Sun1η, we used an additional primer set specific for the Sun1η isoform.

9 Two additional primer sets were used for type-specific amplification: primers 6-1/6-2 6-1/6-2 6-1/6-2bp PCR product only from DNA of HPV types 6 and 11, and primers 16-1 and 16-2 generate a 1413bp PCR product only from DNA of HPV types16.

Additional primer sets were designed for the other insertions analyzed.

To confirm that the VPaI-6 regions examined by PCR assays were contiguous within each isolate, overlapping PCR was carried out using additional sets of primer pairs (Table 3).

An additional primer set from the NSP2 gene of the highly pathogenic vaccine strain JXA1-R was used to detect its exclusive single nucleotide polymorphism.

qPCR was performed as previously described [ 50] using different primer sets (Additional file 5: Table S1).

The reactions were incubated at 42°C for 60 min. The cDNA was acquired from 2 samples and was amplified by PCR using the primer sets (Additional file 6: Table S5).