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Because this TIS site has not been verified using additional experimental approaches such as primer extension assays, we considered this as a putative TIS site.
Therefore, these results should be further validated using additional experimental approaches in order to design a more comprehensive activity model of Padma 28 that reflects also low-dose and long term effects.
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Additional experimental approaches using different members of the ACRATA domain-containing families are required to confirm these hypotheses.
These include the interaction detection method, whether the interaction was detected in vitro or in vivo, on additional experimental approaches used to validate the interaction, and whether the interaction has been independently reported by other research groups.
Several additional experimental approaches were used to confirm this possibility, as described below.
We next aimed to confirm our data in another hepatic cell line (HepG2) using a luciferase promoter assay as an additional experimental approach to determine the influence of IFN-λs on the transcriptional regulation of these two antiviral genes.
Using additional regressors to model activity during the late anticipation phase would further reduce experimental power.
We tested this hypothesis using two experimental approaches.
Studies using other experimental approaches may repudiate/strengthen our findings.
SNPs between A. tridentata subspecies were directly validated using two different experimental approaches: subsequent Sanger re-sequencing of cDNA amplicons (primers listed in Additional File 5) and by re-sequencing targeted loci by sequence capture.
We previously identified testis-/testis cancer-specific splice variants using bioinformatic and experimental approaches [20].
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