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In the present study, high-yield ethanol production was achieved by short-term liquefaction and fermentation of lignocellulose biomass in a novel drum-type rotary fermentation system using a yeast strain developed for cell-surface display of fungal endoglucanase, cellobiohydrolase, and β-glucosidase.
Using a yeast strain with deletions of both HMG1 and HMG2 genes (i.e. completely devoid of HMGR activity) with introduced wild-type or mutant form of human HMGR (hHMGR) gene we investigated the effects of statins on the lipid metabolism of the cell.
This is phenotypically monitored using a yeast strain that has the ade1-14 allele with a suppressible nonsense mutation [ 26, 42].
The S. cerevisiae Holoenzyme was purified as an intact complex using a yeast strain in which both endogenous Mediator and RNA Pol II were affinity tagged.
Using a yeast strain lacking the orthologous gene, YFH1 (yeast frataxin homolog) (Foury and Cazzalini, 1997), a number of potential compounds for the treatment of FRDA that function via an as-yet-unknown mechanism were identified (Cotticelli et al., 2012).
Indeed, releasing Ndt80 from the control of Sum1 by using a yeast strain containing an inducible Ndt80 (MK-ER-Ndt80 strain) results in losing most of the late meiosis repression in some of the Ndt80 targets.
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To find out where Cue1p acts, Sommer and his colleagues used a yeast strain that makes a deformed protein.
In brief, we used a yeast strain that is prone to vesicle production due to an engineered defect in secretory-vesicle/plasma-membrane fusion.
Today's lager brewers use a yeast strain called Saccharomyces carlsbergensis, which scientists have long known to be a hybrid between a classic beer strain called S. cerevisiae and another species that had never been identified in the wild.
We used a yeast strain in which an unrepairable DSB at the mating-type (MAT) locus can be synchronously generated by continuous expression of the endonuclease HO from the galactose-inducible GAL1 promoter, and in which the deletion of the donor sequences HML and HMR prevents the repair of the DSB by HR (Figure 3A; [3A]).
We expressed the ORF CC1225 in the yeast Saccharomyces cerevisiae and used a yeast strain expressing the gene coding for Zm GFOR as a reference.
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