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The activity of these compounds as inhibitors of p53 MDM2 interaction was investigated using a yeast phenotypic assay, herein developed for the initial screening.
Arnoldo A, Curak J, Kittanakom S, Chevelev I, Lee VT, Sahebol-Amri M, Koscik B, Ljuma L, Roy PJ, Bedalov A, Giaever G, Nislow C, Merrill AR, Merrill RA, Lory S, Stagljar I. Identification of small molecule inhibitors of Pseudomonas aeruginosa exoenzyme S using a yeast phenotypic screen.
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In parallel experiments, we used a yeast two-hybrid system to assess phenotypic differences in AHR function.
Sucrose and yeast influenced survival in a complex manner that was best visualised using a phenotypic landscape metaphor.
Performance efficacy of duplex PCR was then compared with phenotypic identification using a standard battery of phenotypic tests.
The method uses a tiny yeast bead with a chemical coating derived from seaweed.
This screening often uses a phenotypic biological end point.
SOURDOUGH -- Bread that has been allowed to ferment naturally, using a sourdough starter or wild yeast, instead of being hurried along with a commercial yeast.
Relocation of the yeast cytoplasmic linear plasmids into the nucleus was assayed using a monitor plasmid pCLU1 in terms of the phenotypic change of the integrated markers (the UCS-fused LEU2 and the native URA3) in S. cerevisiae (leu2 ura3).
Whether using a mixer or not put the water and yeast in the bowl.
Yeast cell number was counted using a hemacytometer.
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