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Insufficient protein yield was also the problem when using a yeast expression system (data not shown).
By using a yeast expression system, mouse PAPST1 and PAPST2 proteins were shown to have PAPS transport activity with an apparent Km value of 1.54 µM or 1.49 µM, respectively.
The substrate specificity of the mouse PAPST1 and PAPST2 proteins was examined using a yeast expression system in a manner similar to that used to investigate human PAPST1 and PAPST2 [31], [31].
During preparation of this manuscript, Luttermann and Meyers (2009) published a similar, more sophisticated, investigation of FCV motif 1 18S rRNA interactions using a yeast expression system in which both mRNA and rRNA partners could be modified.
The transcriptional activity of the CaHsfA2 protein was examined using a yeast expression system.
The biochemical characterization of plant cytochrome P450s using a yeast expression system [ 29] is often difficult due to the low abundance and instability of these membrane-bound proteins.
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In this study we used a yeast expression system described earlier [ 11].
Five C. gibbosum CYP4 sequences (CYP4BK1, CYP4BK2, CYP4BL1, CYP4BL3, and CYP4BL4) were expressed using a yeast heterologous expression system (see Additional file 7).
We first explored the relationship between gene expression profiles and known motifs using a yeast gene expression dataset related to the cell cycle [ 18] and a set of known motifs (Table 1) extracted by AlignACE [ 9].
Using a yeast heterologous expression system for the human androgen receptor, Riggs et al. [ 17] demonstrated that a L119P mutation in the first FKBP domain (FK1) of human FKBP51 yielded a 3.5-fold increase in reporter gene expression.
A cDNA encoding the mature peptide was used to express recombinant MIH (rMIH) using a yeast (Pichia pastoris) expression system.
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