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The transactivation ability of MtCBF4 was analyzed using a yeast assay system.
Firstly, we examined whether these selected SlbZIP proteins had transactivation activity using a yeast assay system.
Furthermore, we also examined whether the SlSR1 and SlSR3L proteins had transactivation activity using a yeast assay system.
Using a yeast assay, we also observed that ABT-737 (that was inactive on the S62A-Bcl-xL or S62D-Bcl-xL expressing control yeasts) was significantly more toxic in S62D-Bcl-xL-transformed than in S62A-Bcl-xL-transformed Bax-expressing yeasts (Supplementary Figure 4B).
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The activity of these compounds as inhibitors of p53 MDM2 interaction was investigated using a yeast phenotypic assay, herein developed for the initial screening.
In a study using dose-dense epirubicin-cyclophosphamide, TP53 mutations were detected using a yeast functional assay.
Using a yeast transcriptional assay, we find significant differences in light sensitivity and fold-activation levels between the red light regulated systems but similar responses between the CRY2/CIB and TULIP systems.
The interaction between VP51A and VP19 was also investigated using a yeast two-hybrid assay.
Using a yeast two-hybrid assay, we showed that zDia2 interacts with constitutively-active RhoA and Cdc42.
We screened a number of PFR proteins identified in our comparative proteomics analysis [18] for interactions using a yeast two-hybrid assay.
When we screened the Ci-binding proteins using a yeast two-hybrid assay, we have also identified importin α3, in addition to Rdx (see Materials and methods).
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