Sentence examples for using a yeast Pichia from inspiring English sources

A cDNA encoding the mature peptide was used to express recombinant MIH (rMIH) using a yeast (Pichia pastoris) expression system.

For example, an interesting collaboration between our Institute and the Seattle-based biotech company, Alder Biopharmaceuticals, led to a new method of using a yeast, Pichia pastoris, to make fully functional whole antibodies more rapidly and cost-effectively than other technologies.

The Mut+ phenotype of Pichia pastoris recombinants was confirmed by PCR with 5′AOX and 3′AOX primers using DNA from the Pichia pastoris transformant as the template that was extracted using a Yeast Genomic DNA Extraction kit (Tiangen, Beijing, China).

Both recombinant proteins were produced using the yeast Pichia pastoris as an expression host, and purified.

The amino acid sequence of paramyosin of S. japonicum mainland strain (Genbank accession number: AAD29285) was translated into a DNA sequence using yeast (Pichia pastoris) codon usage [ 19].

We have examined the feasibility of using the methylotrophic yeast Pichia pastoris to develop a chimeric vaccine candidate displaying the dengue virus type-2 (DENV-2) envelope domain III (EDIII), implicated in host receptor binding and in the induction of virus-neutralizing antibodies, on the surface of non-infectious virus-like particles (VLP -based on the Hepatitis B VLP -based antigen (HBcAg).

Due to their multimeric nature and the requirement of forming disulfide bonds for the generation of functional antigen-binding sites in the scFv domains, trimerbodies have been produced in eukaryotic cells until now, using yeast (Pichia Pastoris) and mammalian cells (Sánchez-Arevalo Lobo et al. 2006; Cuesta et al. 2009, 2012; Blanco-Toribio et al. 2013, 2014).

Airtight storage of moist feed grain using the biocontrol yeast Pichia anomala as biopreservation agent can substantially reduce the process energy for grain storage.

In this study, we used the well-known yeast Pichia pastoris as host for recombinant HRP production.

The activity was characterized using a recombinant protein produced by Pichia pastoris.

We demonstrate here that this process is effectively modeled by a two protein component system using purified lysyl oxidase from the yeast Pichia pastoris to facilitate the oxidation and subsequent cross-linking of recombinant human tropoelastin.