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The yeast was cultured in 500 mL Erlenmeyer flasks using a working volume of 200 mL.
All cultivations were performed in 1 L fully instrumented and automatically controlled BIOSTAT® Q plus fermenters (Sartorius Stedim Biotech S.A, Germany) using a working volume of 1 L.
Cultures of E. coli were performed in 1-L stirred tank bioreactor (Applikon Biotechnology, Schiedam, the Netherlands) using a working volume of 0.35 L.
The experiments were performed in the 2 L Labfors fermentors for 120 h using a working volume of 1.2 or 1.0 L (Table 5).
In routine culture and batch experiments, cells were inoculated at a cell concentration of 2 × 10 cells/mL, using a working volume of 35 mL in 125-mL Erlenmeyer shake flasks (Corning).
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The data that are displayed in Tables 1 and 2 helped to the decision of using a working range from 1.5 to 15 µg/L perchlorate and an injection volume of 750 µL.
These SL analogues are designed using a working model.
Precultured cells were inoculated at an OD600 of 0.2 in three to four individual bioreactors (Innova Biosciences, Cambridge, UK) using a final working volume of 1.5 L.
The actual testing was performed in plates with 96flat-bottomflat-bottomh, using a final working volume of 200 μL per well.
Bellco spinner flasks (100-ml) were used in the experiments for a working volume of 50 ml.
One CSTR (continuous-stirred tank reactor) with a total volume of 40 L and a working volume of 31 L was used for the semi-continuous fermentation experiment.
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