Sentence examples for using a whole transcriptome from inspiring English sources

To explore the role of TLX in inflammation, we assessed changes in the transcriptional landscape of the hippocampus of TLX knockout mice (TLX−/−) compared to wildtype (WT) littermate controls with and without intrahippocampal injection of IL-1β using a whole transcriptome RNA sequencing approach.

Therefore, we performed quasi-random RT-PCR amplification using a whole transcriptome amplification (WTA) kit, according to the manufacturer's protocol with modifications, i.e. 70 cycles of PCR [21].

In order to better define the role of the IL-6 cytokines in the gp130 knockout mouse model, we analyzed gp130 dependent gene expression in isolated hepatocytes using a whole transcriptome approach.

Using a whole transcriptome sequencing technique (RNA-Seq), we were able to identify the levels of differentially expressed genes and establish genes with alternative promoter usage and splicing patterns that changed in association with neurodegeneration.

We occasionally used a whole transcriptome amplification kit (Rubicon Genomics Inc, Ann Arbor, MI, USA) instead of the WGA kit because both kits yielded similar amplification results.

In the present study, we performed a gene-based SNP discovery analysis in bovine Longissimus thoraci, using a whole-transcriptome sequencing approach.

Increasing sampling effort just before the onset of hatch to the point of first exogenous feeding, and using a whole-transcriptome approach, could prove fruitful.

Therefore, in an attempt to investigate the complex landscape of mutations, changes in expression profiles and alternative splicing (AS) events that can be observed in such disease, the leukemia transcriptome of a BCR ABL1-positive ALL patient at diagnosis and at relapse was sequenced using a whole-transcriptome shotgun sequencing (RNA-Seq) approach.

Using a whole root transcriptome approach, a detailed analysis of root mRNA profiles of contrasting grapevine genotypes is presented.

To our knowledge, buccal cells have not been used in a whole transcriptome approach to investigate differential gene expression between smokers and nonsmokers using concurrently harvested samples in a manner which directly compares expression differences.

The Synechococcus sp. PCC 7002 RNA-Seq data were generated from an 0.5 μg RNA sample using a SOLiD™ Whole Transcriptome Analysis Kit (Applied Biosystems) and the SOLiD™ 3Plus protocol as described previously [ 28].