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Whole slides were scanned at a maximum resolution of × 20 using a whole slide scanner (NanoZoomer 2.0, Hamamatsu Photonics, Hamamatsu City, Japan).
For digital IHC quantification, slides were scanned at 40 × magnification (0.25 microns/pixel) using a whole slide scanner (ScanScope CS, Aperio Technologies, Vista, CA, USA) fitted with a 20x/0.75 Plan Apo objective lens (Olympus, Center Valley, PA, USA).
TMA slides were scanned at 40 × magnification (0.0625 μm/pixel) using a whole slide scanner (ScanScope CS or ScanScope XL; Aperio ePathology, Leica Biosystems, Vista, CA) and preprocessed using the Genie Histology Pattern Recognition software suite (Aperio) to segment tissues into three user-defined Image Classes (tumor, stroma, glass) as previously reported [ 28].
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High-resolution digital images of each TMA were generated using a whole-slide scanner (SanScope XT automated high-throughput scanning system) from Aperio (Vista, CA).
All IHC-stained slides were digitised and analysed using a whole-slide imaging system (ScanScope XT; Aperio Technologies; San Diego, CA, USA; Table 3).
Slides were scanned using a whole-slide imaging system (ScanScope XT, Aperio Technologies, Vista, CA) and annotations were drawn on whole-slide images using a pen tablet screen to exclude areas not representative of AF fibroblasts/myofibroblasts, including large vessels and artifacts such as tissue folds.
When performing variance analyses on DNA-microarray data, one can either use a whole-slide model or a more complicated model that allows for gene-to-gene differences.
To do this, a whole slide image was used to produce a supported image by the morphological operation of opening and brightness equalization.
Use a whole milk wash.
(Some people use a whole room).
Use a whole house surge suppressor.
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