As the reduced number of pollen grains in ashh2 could also be due to defects in male meiosis, we inspected meiosis in ashh2-1, andh2-2 and ashh2-5 lines using a whole-mount DAPI/Aniline Blue staining procedure.
We are currently undertaking full cDNA insert sequencing for a set of non-redundant clones, as well as characterizing their expression using a whole-mount in situ hybridization screen [ 28, 29].
Therefore, we measured apoptosis using a whole-mount TUNEL assay (Pellettieri et al., 2010) and observed a remarkable increase in cell death throughout the course of egr-5 RNAi treatment compared to control animals.
Representative transgenic lines were assessed using a whole-mount immunolocalization technique after Sauer et al. [ 18] to confirm recombinant protein localization in the target (endoplasmic reticulum, apoplast and vacuole) area and, as shown in Figure 2C, expression of genes was confirmed in the targeted plant cell compartment.
The samples mathematically analysed using the whole mount technique were from the third generation bronchi.
The key for identifying these meningeal lymphatic vessels by both labs was the use of a whole-mount preparation where the meninges were fixed while still attached to the skull, thus enabling the preservation of fine meningeal vessels.
We examined the expression of Gadd45γ, which encodes a known MAP3K4-interacting protein, using whole mount in situ hybridization (WMISH).
This is illustrated in Figure 1B using whole mount in situ hybridisation with an fmr1 specific probe, on a batch of embryos obtained from a cross between heterozygous parents.
We performed immunoelectron microscopy using "whole mount" samples and cryo-immunoelectron microscopy on strains F. tularensis SCHU S4, 1547 and LVS using MAb 11B7.
Dopaminergic neurons were first counted at 5 dpf using whole mount in situ hybridization (WISH) staining with a probe for tyrosine hydroxylase (TH) (n = 10 embryos per genotype and biological replicate).
