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Pressure transducers were calibrated using a water column.
The device was calibrated using a water column, and a pressure sensitivity of 1.636 × 10−2 MPa−1 was yielded.
A similar model of leakage, which is greater during inspiration than during expiration, were used by Carteaux et al., using a water column of variable height to study the frequency of asynchronies at different leak levels [ 12].
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The resulting solution (20 μL) was analyzed by Waters HPLC 515 having Rheodyne 7725i injector fitted with 20 μL loop and equipped with photodiode array (PDA) 2998 detector (Waters, USA) using a Waters column, C18 spherisorb 5.0 μm ODS2 4.6 mm × 250 mm column.
Amino acid analysis was performed using a Waters AccQTag column.
Reverse phase separation was performed at 30°C using a Waters C18 column (3.9 X 150 mm) (Waters, USA).
Semi-preparative HPLC was carried out using a Waters C18 column (15 μm, 100 Ǻ, 7.8 × 300 mm).
Samples were subsequently analyzed by nanoUPLC-MS/MS using a Waters, nanoAcquity column, 75 μm × 250 mm, 1.7 μm particle size, and a gradient of 1 40% acetonitrile in 60 min at a flow rate of 250 nL/min.
According to study the high-performance liquid chromatography (HPLC) analyses were carried out on a normal phase column or reverse phase column using a Water 2998 photodiode array detector (260 370 nm) and an isocratic system of elution or on a chiral phase column.
The UPC loaded silica was dried by drawing air through the column using a water aspirator.
HPLC procedure Latrunculol A in the eluted fraction was determined using a Waters Symmetry NH2 column (3.9 × 300 mm) with a 10-μm particle size and a pre-column of the same material as the stationary phase.
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