The degree of lymphedema in our animal model was measured by volumetric analysis using a water displacement method.
Quantification of retinoid levels was carried out by HPLC analysis using a Waters 2690 Separations Module and 996 Photodiode array detector (Waters Ltd ,Elstree, UK) and Waters Millennium software for data acquisition.
Samples were reconstituted in 200 μl mobile phase A and intra- and extracellular retinoid levels were quantified by HPLC analysis using a Waters 2690 Separations Module and 996 Photodiode array (PDA) detector (Waters Ltd., Elstree, UK), with Waters Millennium software for data acquisition, as described previously (Veal et al, 2002).
MS analysis was performed using a Waters xevog2qtof mass spectrometer.
UPLC/Q-TOF MS analysis was performed UPLC/Q-TOF MSs analysisUPLC system composed of a binary solvent manager and a photo diode array detector (203 nm).
Amino acid analysis was performed using a Waters AccQTag column.
HPLC analysis was performed using a Waters 2695 separation module (Waters, Milford, MA, USA).
HPLC analysis were conducted using a Waters system (Waters Co., Milford, MA, USA) with a 2424 ELS detector and a 1525 binary HPLC pump.
Sample analysis was performed using a Waters ACQUITY UPLC system (Waters Corp., Milford, MA, USA), and a Waters Xevo triple quadrupole tandem mass spectrometer (Waters Corp., Manchester, UK) was used to detect the analytes.
MRM analysis was performed using a Waters Xevo TQ mass spectrometer (Waters, MA, USA) coupled to a Waters nanoAcquity UPLC via a Zspray Nanoflow source with a 10 μm SilicaTip PicoTip emitter (New Objective, MA, USA).
