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The core body temperature of the mice was maintained using a warming pad set to 37 °C.
Esophageal temperature was monitored and maintained at 36-37 °C throughout the operation using a warming pad.
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Why not put it to use as a warming pad?
Protective eye lubricant (VitA-POS, Ursapharm, Saarbrücken, Germany) was administered to both eyes, loss of body temperature was minimized using a warm water heating pad and supplemental oxygen was provided through a nose cone.
Apply heat either with a heating pad or by using a warm whirlpool bath if one is available after a day or two, once the acute pain calms down.
All mice were kept warm using an infrared warming pad (Kent Scientific) placed under and around the bed while under isoflurane anesthesia in the microPET/CT scanner (pixel size 200 μm).
The blood temperature, determined by means of the pulmonary artery catheter, was maintained at 37.2 ± 1.1°C during the experiment using an infrared warming lamp and a warming pad.
A warming pad was used to keep body temperatures around 37 °C during the surgery.
Mice were anaesthetised with 1.5 % isofluorane/oxygen, placed on a warming pad, and imaged in the supine position using a Vevo770 ultrasound system with high-frequency 45 MHz RMV707B scanhead (VisualSonics, Amsterdam, The Netherlands).
Briefly, mice were anesthetized using tribromo-ethanol (Avertin) and transferred to an imaging stage equipped with a warming pad for controlled maintenance of mouse body temperature at 37°C.
After anesthesia using ketamine (15 mg/kg, intramuscular injection) and inhalation of 1.5% isoflurane, the male minipig (Livestock Research Institute, Taitung, Taiwan), weighing 16 19 kg, was placed in supine position on a warming pad.
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