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RNA extraction using a volume of 500 µl was useful to improve sensitivity for low VL samples.
Numerical simulations using a volume of fluid (VOF) approach were performed for a vertical falling film arrangement.
To explore the laterality of the effects further, we performed an additional ketamine>controls and controls>ketamine analysis restricted to either the left or the right hemisphere using a volume of interest.
The transient calculations were carried out using a Volume of Fluid (VoF) multiphase flow model of the commercial CFD code of FLUENT 6.3.
In this numerical method, the large bubble is tracked using a volume of fluid (VOF) method, and the small bubbles are tracked using a discrete particle method (DPM).
Therefore, in this work, after proposing a two-dimensional model, we focus on the interface tracking using a volume of fluid method.
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Complex solid boundaries are represented using a volume-of-fluid approach.
The motion of the interface is captured using a volume-of-fluid method.
In this work, droplet-droplet interaction is modelled using a multiscale approach which couples multiphase flow simulation using a volume-of-fluid method to a surface thin-film model operating on the sub-grid scale.
Acquisition time was approximately 30 min. The CT and SPECT reconstruction was performed using InVivoScope 1.37 software (Bioscan) and images were quantified using a volume-of-interest (VOI) approach.
That means every year we use a volume of water representing almost seven cubic kilometres.
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