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Even if you follow the most complex steps the rigorous Mr. Fremer demonstrates – like using a volt meter to balance the left and right channels – he acknowledges the impossibility of achieving perfection.
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If you want to know how to use a volt meter to do this, read this article.
Figure 5 compares DTPA absorption to TER as measured in individual cell cultures on the day prior to DTPA absorption measurements, using a volt-ohm meter.
TER was also measured in a series of individual cell cultures on the day prior to measurements of DTPA absorption, using a volt-ohm meter.
Measurements of TER were also performed using a volt-ohm meter to determine the relative effect of tight junction permeability on DTPA absorption from individual cell cultures.
Measurements of TER from individual cell cultures were made using a volt-ohm meter 24 h prior to the addition of 10 μL of Tc-DTPA in PBS; one CF line (n = 12) and four non-CF lines (n = 30).
Comparisons of DTPA absorption after benzamil addition, TER values for individual CF and non-CF cell cultures (made using a volt-ohm meter), DTPA absorption rates with and without apical mannitol, and measurements of TER with Ringer's vs. Ringer's + mannitol (by Ussing chamber) were also performed by unpaired t test.
Transepithelial electrical resistance was measured using a volt-ohm meter (World Precision Instruments).
TEER was measured in both P0 and P1 EBEC ALI-cultures as an index of tight junction and epithelial barrier function, using a volt-ohm-meter (Millicell®-ERS, Millipore Corp., Billerica, MA, USA).
Transepithelial electrical resistance (TER) was measured using a volt ohmmeter.
Try using a Volt Shroom at the beginning of the battle.
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