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Following immunolabeling with anti-TH antibody, sections were scanned at 4× magnification using a virtual slide microscope scanning system (VS110, Olympus) and quantified using NIH ImageJ software.
For the NET data, the Haematoxylin and Eosin (H&E) stained sections, coregistered with the MALDI-imaging results, were evaluated histologically by an experienced pathologist using a virtual slide scanner (MIRAX desk, Carl Zeiss MicroImaging GmbH, Munich, Germany).
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Slides were also independently digitally scanned using a Virtual Microscope ScanScope Unit and ScanScope Console program at 200 × magnification, and visualised using Image Scope (Aperio Technologies, Vista, CA).
The sliding spotlight mode allows a comprise between azimuth resolution and azimuth extension of imaged scene [1 3], and it can be described as using a virtual rotation center which is further away from the radar than the imaging scene [4].
The slides were photographed with the use of a virtual slide system (Scanscope CS system, Aperio Technologies, Vista,CA).
We start with image segmentation in a virtual slide, find specific patterns and use a set of features which can describe pseudopalisading necrosis and distinguish it from palisades.
Stained slides were scanned with a virtual slide scanner (NanoZoomer 2.0-HT, Hamamatsu, Japan) and viewed using NDP viewer (NanoZoomer Digital Pathology).
Using software that can insert any number of sampling points within a systematic grid onto a virtual slide (Treanor et al, 2008), scores can be entered and saved as an electronic file for further analysis.
Digital pathology promises a number of benefits in efficiency in surgical pathology, yet the longer time required to review a virtual slide than a glass slide currently represents a significant barrier to the routine use of digital pathology.
The pictures can be organized into different albums and displayed in a virtual slide show.
Scanning a glass slide with the array microscope produces seamless two-dimensional image data of the entire slide, that is, a virtual slide.
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