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We observe that intrastriatal delivery of NGF and BDNF using a viral vector system can mitigate, albeit only moderately, neuronal death following stroke, which leads to detectable functional sparing.
Using a viral vector that Schaffer developed for gene therapy, the team inserted genetic blockers into the brains of old mice to knock down TGF-beta1 activity, and found that hippocampal stem cells began to act more youthful, generating new nerve cells.
Two different shRNAs against Sec61β were individually introduced into COS-7 cells using a viral vector.
Thus, an effluc reporter gene was additionally adopted for tracking the migration of DCs and dual reporter DCs expressing both the NIS and effluc genes were successfully established using a viral vector system expressing the reporter genes.
Although it's possible to deliver CRISPR's components into cells in a dish as proteins or RNA, so far researchers can usually only get it working in tissue inside the body by using a viral vector to deliver the DNA for Cas9 into cells.
The three teams, headed by Yamanaka, the Massachusetts Institute of Technology's Rudolf Jaenisch, and Harvard's Konrad Hochedlinger, all began by following Yamanaka's procedure, using a viral vector to introduce copies of genes for four transcription factors active in ES cells: Oct4, Sox2, c-Myc, and Klf4.
Similar(45)
This system uses a viral vector, RCAS, derived from the avian leukosis virus (ALV) [76].
Even though we did not use a viral vector, the presence of the strong viral enhancer/promoter is still a potential concern.
More recently, heterologous prime-boost regimens mainly use a viral vector or a DNA vaccine for priming, followed by a boost with a protein-based vaccine.
Rosselló et al. used a viral vector that carries the four stem-cell genes (from the mouse) into target cells from the different species.
Suckau et al. successfully used a viral vector expressing optimized miR-mimics in mice to normalize cardiac dilation and significantly reduce cardiac hypertrophy and cardiac fibrosis [ 106].
More suggestions(15)
using a viral suspension
using a viral viral
using a lossless vector
using a viral co-infection
using a viral extraction
using a random vector
using a viral approach
using a viral strain
using a viral suppressor
using a retroviral vector
using a structural vector
using a lentiviral vector
using a viral microneutralization
using a viral titer
using a viral amplification
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