Sentence examples for using a viral suspension from inspiring English sources

Virus inoculation of cell cultures was performed using a viral suspension containing homogenized infected mouse brains at a final concentration of 1∶100 (v/v) in culture medium.

Viruses were added while cells were in single-cell suspension using a viral MOI of 5. Medium was doubled the following day and cells were transferred to a 12-well plate at 4 days after transduction and expanded for further 6 days before differentiation.

Viral RNA was extracted from 140 μL of the resulting suspension by using a Viral RNA Minikit (QIAGEN, Mississauga, Ontario, Canada) and eluted in a volume of 50 μL.

Nucleic acid was extracted using a viral nucleic acid extraction kit (Geneaid, Taiwan) from 200 μL of 10% fecal suspension to a final volume of 50 μL of RNase-free H20.

Then, the animals were anaesthetised (ketamine/medetomidine) and a viral suspension containing LVV-EF1α-TMPAP EGFP LVV-EF1α-TMPAP EGFPmicroinjected biLVV-EF1α-TMPAP EGFPRVLM using stereotaxic coordinates indicated above.

Standard injection was 2 µl of a viral suspension containing 10E8 transducing units (t.u).u

The lowest dilution of a viral suspension at which serum samples still inhibit agglutination is determined by visual inspection and indicates neutralization titer.

Template RNA was extracted from patient fecal suspensions using a QIAamp Viral RNA Mini Kit (Qiagen, Germany) following the manufacturer's instruction.

Tail Suspension was performed using a Mouse Tail Suspension setup (Med Associates, Georgia, VT).

RNA was extracted from the brain suspensions of the mice using a QIAamp Viral RNA Mini Kit (QIAGEN, Germany, Hilden) according to the manufacturer's instructions.

Viral RNA and DNA were extracted from stool suspensions clarified by centrifugation (1500 × g, 20 min) using a QIAamp® Viral RNA Mini kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol.