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A direct comparison of Nef accumulation levels in agroinfiltrated leaves using a viral suppressor of gene silencing and transgenic plants cannot be made, nevertheless, we showed that transient expression using AMCV-P19 is an advantageous system for rapid and high yield production of Nef.
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Emerging viral infections can be identified by using a viral metagenomics approach for clinical human material.
In all cases, progeny carrying a viral suppressor transgene exhibited the developmental phenotype associated with expression of the suppressor in the parental line (data not shown).
Viral RNAs were extracted from the viral isolates using a QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA) and stored at −80°C for further use.
Viral RNAs were extracted from pelleted virions using a QIAamp Viral RNA Mini Kit (Qiagen).
Mathematical modeling was performed using a standard viral dynamic model.
Taken together, the results presented here suggest that the use of viral suppressor of gene silencing, AMCV-P19, could efficiently enhance the transient expression of recombinant proteins in N. benthamiana.
Our results demonstrate the effectiveness of using viral silencing suppressor proteins as a tool to dissect small RNA pathways in animals, as previously established in plants.
To overcome this limitation, the use of viral suppressors of gene silencing in the agroinfiltration assays was able to prevent PTGS and enhance transient expression levels of heterologous proteins.
Use a suppressor, this will conceal you to enemies.
To monitor viral fusion events, we used an established viral fusion assay [35].
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