Therefore, we used a viable cell counting kit (CCK-8 kit) to measure viable cells encapsulated in the peptide hydrogels, which is similar to the method described by Kisiday et al. [ 15].
Assessment of viable and non-viable cell numbers was performed using Trypan Blue exclusion counted using a haemocytometer; viable cells exclude the dye.
We found that regulating TGF-β1 had a significant effect on the proliferation rate of oral cancer cells determined using direct viable cell number counting, and cell proliferation in situ, detected using a BrdU FITC kit.
Cell viability was measured by determining whole cell numbers using a haemocytometer, and viable cells were excluded using trypan blue (Gibco, Paisley, UK).
Cell enumeration was carried out using a hemocytometer, and viable cells identified by trypan blue exclusion.
Ten microlitres from this solution were used to count the viable cells by using a Countess automated cell counter (Invitrogen, UK).
Briefly, cells were stained with 0.4% trypan blue (Life Technologies Inc., Grand Island, NY, USA) and viable cells counted using a Countess automated cell counter (Life Technologies Inc .. Seven siRNAs targeting the HPV16 oncogene E7 were chosen for initial testing.
Using viable cell counts and lactate dehydrogenase the release of Hsp72 was shown to not be due to cellular damage [ 60].
Using viable cell counting and the incorporation of radioactive thymidine in cell TCA precipitate, we have attempted to separate the effects of asbestos on cell growth inhibition (reproductive failure) vs. cell killing.
We conducted a binding analysis of rituximab using the viable cells of B-lineage purified from cryopreserved mononuclear cells.
