Sentence examples for using a variance stabilization from inspiring English sources
For both mRNA and miRNA data, we first transformed raw data generated from BeadStudio (Illumina, San Diego, CA) using a variance stabilization transformation algorithm [35] and then normalized them using quantile normalization implemented in Bioconductor (www.bioconductor.org).
Data were transformed using a variance stabilization transformation [ 62], which takes into account the large number of technical replicates on Illumina arrays, and normalized using a robust spline normalization.
The background-adjusted fluorescent values generated by GenePix Pro were normalized for each microRNA using a variance stabilization method described by Huber et al[ 34], followed by a Welch two-sample t-test carried out for every gene; and a multiplicity correction was conducted to control FDR at 5% using a step-up approach, as described by Benjamini and Hochberg[ 35].
The data were normalized using a variance-stabilization procedure (VSN) [ 45].
A second problem is that an accurate noise model is needed in order to build such a variance stabilization transform.
The proposed approach takes into account the photon-limited nature of the images via a variance stabilization transform and in particular for WF microscopy, occurrence of photo-bleaching.
On the other hand, Lin et al. [ 13] proposed a variance stabilization transformation that incorporated bead intensities variation and showed an improvement in differentially expressed gene detection.
The data were transformed using the variance stabilization transformation (VST) [37] and normalized using the robust spline normalization (RSN) [36].
The data were then transformed using the variance stabilization transformation algorithm (VST) and normalized using the quantile normalization method.
Signal was transformed and normalised using the variance stabilization algorithm as implemented in the vsn2 [ 32] Bioconductor [ 33] package.
Gene expression values were obtained by first correcting for the background and normalizing on probe level using the variance stabilization method by Huber and colleagues [ 55].
