Sentence examples for using a vapour diffusion from inspiring English sources

Crystallisation was performed using a vapour diffusion technique at 21°C with 0.5- to 1-μl hanging drops in EasyXtal plates (QIAGEN, Valencia, CA, USA).

The ethanol oxidation activities of catalysts 1– 5 were studied using a vapour-phase fixed-bed reactor.

It uses a vapour off-take system, which uses a cylinder full of liquid gas.

Screens were set up using commercially available crystallisation kits, using vapour diffusion, with drops consisting of 500 nl protein solution and 500 nl reservoir.

In contrast to the Insugen (I) crystals, Intergen (II) crystals were grown using the vapour diffusion hanging drop method at 293 K.

All crystallization experiments were carried out at 293 K using the vapour diffusion method.

Initial screens of the entire CF1FO-ATP synthase at a concentration of 18 mg/ml were set up using the Memplus screen from Molecular Dimensions, UK, using the vapour diffusion method.

In order to determine the conditions for crystallization, each protein was initially screened using sitting-drop vapour diffusion with a bespoke 96 condition sparse matrix screen buffered at 6 different pHs using the multi-component buffer PCTP (Newman, 2004; Zhang et al., 2013).

Optimized crystals of selenomethionine-labelled methylated SsoCas6 were grown under conditions of 0.05 M bicine (pH 9.1) and 28% PEG [poly ethylene glycol)] 3350 using vapour diffusion with a protein/precipitant ratio of 1 1.

Human Parkin UPD-RBR was crystallized at a concentration of ∼4 mg/ml using vapour diffusion.

For crystallisation experiments Chd1-DBD was exchanged into 20 mM Na Cacodylate pH 7.1, 100 mM NaCl, and 1 mM DTT. Native and SeMet crystals were grown at 20°C using hanging drop vapour diffusion by mixing a 20 mg/ml protein solution with reservoir solution (0.1 M Tris pH 8.5, 28 30% PEG 4000, 0.35 0.45 M MgCl2) at ratios of 1 2 0.5 2.5.