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Exposures Trained observers evaluated delirium daily using a validated protocol.
Using a validated protocol, renal tissue samples were prospectively collected from patients undergoing nephron-sparing or radical nephrectomy.
MDA-MB-361 lapatinib-resistant (LR) cells were generated by using a validated protocol of in vivo/ in vitro selection after prolonged exposure to the drug [ 24].
DNA was extracted and amplified at Roche Molecular Systems prior to being sent to a CLIA-certified laboratory (SeqWright, Houston, TX, USA) to be sequenced using a validated protocol for EGFR mutation detection.
Blood pressure is measured by using a validated protocol [ 64] at the right brachial artery in the fossa cubitalis after the subjects rest for 5 min in a supine position.
Blood pressure is measured at the right brachial artery in the fossa cubitalis after the children have been resting for 5 min in supine position by using a validated protocol [ 33].
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Blinded whole-blood samples were transported, using a validated shipping protocol, overnight in a blinded fashion to the University of Washington, Seattle, WA, for T-cell analysis (10, 11).
In accordance, PRL expression did not reach detectable levels in any of the 19 human breast carcinomas or 5 cell lines, which were analysed using a validated ISH protocol.
The selection of indicators provided by the experts needs to be confirmed by data collection specialists and this then needs to be tested in practice using a validated testing protocol [ 6, 20].
MIF amounts in rat intraluminal fluid were detected using a validated, custom ELISA protocol for detecting rat and mouse MIF developed in our laboratory and described previously [6].
Histological analyses on thymic samples were used following a validated protocol to classify samples as positive, suspect or negative for corticosteroid treatment and results have been reported previously [ 17], showing a relevant percentage of suspect animals for corticosteroid treatment compared to official data.
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