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Random blood samples (specifically timed in relation to the dose administered) were obtained, and the serum concentrations of polymyxin B1 were assayed using a validated methodology by liquid chromatography mass spectroscopy.
The concentration of melatonin released at each time was determined by HPLC using a validated methodology previously described [24].
Final estradiol concentration was measured by gas chromatography coupled to a mass spectrometry detection system (6890-GC/5973-MSD, Agilent TeCalifornia, California, USA) using a validated methodology.
These were pooled using a validated methodology to account for biological variance (Kendziorski et al, 2005; Zhang et al, 2007) creating three pooled samples of α2 β1HI CD133+VE, α2 β1HI CD133−VE and α2 β1LOW CD133−VE.
Video-tracking and sleep estimation were performed as described previously (Fisher et al. 2012) using a validated methodology which showed a correlation coefficient of more than 94% when compared with electroencephalography (EEG) recordings.
Even though no influence of pubertal stage was identified on prevalence of prediabetes, it is noteworthy that puberty was not assessed by a physician but was self-reported by the study participants using a validated methodology.
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We recently used a validated methodology to measure systemic and renal hemodynamics directly, accurately, and over several days by implanted transit-time flow probes [15]-[17] [15]-[17] thand contrary to expectation, intravenous contrast administration induced only short-lived renal vasoconstriction (found hour) followed by susthated (days) renal vasodilatation and incontraryRBF [18].
This is the first study reporting detailed in-vivo airway cross-sectional area measurements from cricoid to lobar bronchial divisions and area based airway proportion measurements from children using a validated bronchoscopic methodology.
While rich qualitative data are an essential starting point, this preliminary study aimed to pilot a much-needed quantitative and comparative approach using a validated instrument and easily replicable quantitative methodology.
Eight studies used modelling methodologies [ 34, 35, 37- 42].> -wrap-foot> ∆Quality of empirical studies were assessed using a validated tool [ 27].
Formal assessments of body composition were assessed by DEXA using a validated in-house protocol71.
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