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Nutrient intake was analyzed using a validated computer program (DGE-PC 3.0; Deutsche Gesellschaft für Ernährung).
In this sense, virtual simulation softwares, using a validated computer model, will be able to carry out "experiments" under controlled conditions, with realistic data.
The randomization list was prepared centrally by a Contract Research Organization, Max Neeman International Data Management Centre, using a validated computer program (Statistical Analysis Software SASS) 9.1.3, SAS Institute Inc ,Cary, NC) PROC PLAN procedure).
From accelerometer indications of wrist activity, in-bed sleep-wake state was estimated for one-minute epochs, using a validated computer algorithm, supplemented by "Webster's rules" [ 21] and hand-editing, e.g., to delete epochs when the device was removed for bathing.
The cavitated dentin lesions were randomly assigned to one of the treatment groups after stratification for gender, operator, upper/lower jaw and, when needed, according to left/right side of the mouth using a validated computer software program (Trial Balance, Lausanne, Switzerland).
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Retinal vessel caliber was measured from retinal photographs using a validated computer-based technique.
Central retinal arteriolar equivalent (CRAE) and central retinal venular equivalent (CRVE) were obtained using a validated computer-assisted program.
Mammographic density, before diagnosis in cases, was quantified by using a validated computer-assisted method.
Trained observers will identify individual arterioles and venules using a validated computer-assisted program, IVAN (Vasculo-matic Nicola, Ophthalmology and Visual Science, University of Wisconsin-Madison, USA) (17).
Mammographic percent density was assessed using a validated computer-assisted method (Martin et al, 2006; Douglas et al, 2008) and included multiple prospective quality measures to minimise bias.
In addition, we have evaluated retinal fundus images using a validated computer-assisted program and standardized assessment of a range of vascular risk factors.
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