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The amount of proteins eluted was monitored by measuring their absorbance at 280 nm using a UV monitor (Model EM-1 Econo UV monitor, Bio-Rad Laboratories).
Protein concentration was monitored using a UV monitor at 280 nm and a refractive index detector was set at 690 nm (Optilab DSP, Wyatt Technology, Santa Barbara, CA, USA).
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The gradients were fractionated using a piston gradient fractionator (BioComp Instruments, Fredericton, NB, Canada) and UV absorbance at 254 nm was monitored using a UV-Monitor (BioRad, Hercules, CA).
Purification and analysis of products were performed by HPLC Thermo Scientificc, Waltham, MA, USA), and eluates were monitored using a UV detector (218 nm).
Protein elution was monitored using a UV detector at 280 nm.
Chromatographic separations were monitored using a UV detector at 340 nm.
Furthermore, the HPLC separation can be easily monitored using a UV detector allowing for precise fractions to be transferred to the GC.
Hydrolysis was monitored at 480 nm using a UV spectrophotometer (Shimadzu, Japan).
All eluates were monitored at 280 nm using a UV detector.
The supernatant was diluted 5-fold with PBS and was monitored at 415 nm using a UV spectrophotometer.
The reaction progress was monitored using a UV-visible spectrophotometer.
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