LAMP products were examined directly using a UV light and verified by agarose gel electrophoresis.
Subsequently, the DNA-protein complexes in the gel were transferred to a positively charged nylon membrane by electrophoretic transfer in 0.5X TBE at 380 mA for 30 min, cross-linked at 120 mJ/cm2 using a UV light cross-linker, and detected using a LightshiftTM Chemiluminescent EMSA Kit (Pierce).
Therefore, attempts have been made to use the intercalating dye SYBR-Green to measure the end reaction using a UV light [13], [15] or conventional real-time PCR fluorescence readers [19] [22].
Thermal cycling parameters were defined as follows: predenaturation at 95°C for 2 min; followed by 32 cycles of 95°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s; and then a final step at 72°C for 5 min. PCR products were separated on a 2% agarose gel containing ethidium bromide and visualized using a UV light source.
PCR products were visualized on 1.2% (w/v) agarose gels using a UV light transilluminator.
The UV power output was measured using a UV light meter with a peak sensitivity at 340 nm (LUYOR, UV 340B, China).
Later, the gel was visualized using a UV light source and imaged using a Gel Doc XR gel documentation system (Bio-Rad, Hemel Hempstead, UK).
Before treatment, an intended 3.0 mW/cm2 of surface irradiance (5.4 J/cm2 surface dose) was calibrated using a UV light meter at a working distance of 5 cm.
The gel was photographed using a UV light box with an EOS Rebel T3i camera (Canon, Arlington, VA) with a HD UV filter (Canon).
After 35 cycles of PCR, the PCR products were resolved by electrophoresis in agarose gels and visualized by ethidium bromide staining using a UV light source.
