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LAMP products were examined directly using a UV light and verified by agarose gel electrophoresis.
Subsequently, the DNA-protein complexes in the gel were transferred to a positively charged nylon membrane by electrophoretic transfer in 0.5X TBE at 380 mA for 30 min, cross-linked at 120 mJ/cm2 using a UV light cross-linker, and detected using a LightshiftTM Chemiluminescent EMSA Kit (Pierce).
Therefore, attempts have been made to use the intercalating dye SYBR-Green to measure the end reaction using a UV light [13], [15] or conventional real-time PCR fluorescence readers [19] [22].
Thermal cycling parameters were defined as follows: predenaturation at 95°C for 2 min; followed by 32 cycles of 95°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s; and then a final step at 72°C for 5 min. PCR products were separated on a 2% agarose gel containing ethidium bromide and visualized using a UV light source.
PCR products were visualized on 1.2% (w/v) agarose gels using a UV light transilluminator.
Scoring recapture numbers was done on the retrieved Sticky Pads with daily CM catches using a UV light in a dark room.
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UVB irradiation was carried out using a UV lighter (LEITUO illumination, Shenzhen, China).
This group used a UV light (254 nm, 25 W) irradiation method for 30 min and heated for 30 min at 80 °C to obtain 89 nm silver nanoparticles.
Use a UV light and highlighter on your models for rich contrast.
Use a UV light treatment device to kill bacteria inside the shoe daily.
Gels were stained using ethidium bromide (Sigma #46067-50ML-F) and visualized using a UV-light transilluminator.
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