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The reason for using a UV filter is more of a protection for the lens against scratches.
Hoechst stained nuclei were visualized using a UV filter cube DAPI-1160AA, SemRochesterhester, NY) while GFP-MSH6 was visualized using a GFP filter cube (Filter Set 13, Carl Zeiss MicroImaging, Thornwood, NY).
Fluorescence of glutathione-conjugated monochlorobimane (optimal Ex394/Em490) was detected using a UV filter set.
Propidium iodide was detected in protoplasts using a UV filter cube.
For measurement of cell plate angle and apical cell length, plants regenerated from protoplasts were stained with 0.1 mg ml−1 calcofluor solution and visualized by epifluorescence microscopy (Leica MZ16FA, Leica Microsystems, Buffalo Grove, IL) using a UV filter (excitation 360/40 nm, emission 420 long pass) or a Violet filter (excitation 425/40 nm, emission 400 long pass).
FluoroGold-labeled RGCs were counted under an Axioplan microscope (Zeiss) using a UV filter (365/420 nm) per visual field in the central region of the retina (0 1 mm from the optic nerve head along the retina diameter; six visual fields per retina).
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Use a UV filter or a lens hood to protect your lens from sand or loose debris.
Nuclear chromatin was visualized using a UV-2E/C filter (325-375 nm) [ 50].
The gel was photographed using a UV light box with an EOS Rebel T3i camera (Canon, Arlington, VA) with a HD UV filter (Canon).
Hoechst 33342 was excited by a mercury arc lamp using a 450/50 nm band-pass filter, and emission was collected using an UV-2E/C filter (Nikon).
Particularly, using an UV B/C blocking filter, which permits to pass light in the 350 550 nm range, an about two-fold intensification in the current-to-electrical energy conversion (normalized to the same total irradiance) is obtained.
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