this was monitored at an absorbance of 259, 280 and 360 nm each of Rutin [ 30, 31], Catechin [ 30, 32] and Kaempferol [ 33] respectively, using a UV-detector (Photo-Diode Array, Waters Model No. 2996).
Fluorescence microscopy was performed using the Zeiss LSM 510 Meta inverted confocal microscope equipped with a ×63/1.4 NA (numerical aperture) oil objective, multiline argon laser (488, 514 nm), DPSS (diode-pumped solid-state) laser (561 nm) and a UV diode laser (405 nm).
f) Acetonitrile: water (60: 40) g) Acetonitrile: methanol (70: 30) A UV diode array detector was set at five different wave lengths i.e. 230nm, 235nm, 240nm, 245nm and 250nm.
We used an UV-light-emitting diode for fluorescence excitation and a photomultiplier tube (PMT) to record emission.
UV detection was performed using a diode array detector in the 200 400 nm passband, and quantification of the analytes was made at 220 nm.
Sugars were detected by indirect UV detection using a diode-array detector.
They could be observed using an ordinary UV illuminator or UV-LED (light emitted diode).
Lignin autofluorescence was monitored using a 405 nm Diode laser.
For MAQRO, we will include multiple buffer-gas tanks, each with at least one HCPCF leading to the outer loading mechanism (see Sections 7.1.6 and 7.2.6), and we will use redundant UV diodes based on the idea of redundant pump diodes in LTP.
UV spectroscopic analysis was done using an S2000 UV/Vis Diode Array Spectrophotometer, Biochrom machine while melting points were measured on a Stuart Scientific (SMP1) melting point apparatus.
