All reactions were run in triplicate on 96-well plates, using a unique sample as endogenous calibrator control in each one, and analyzed using the Applied Biosystems SDS 7500 system software (Applied Biosystems).
Blood samples were collected by venepuncture in EDTA anticoagulant vacutainers, coded using a unique sample identification code and processed daily within 12 hours, keeping them at 4°C inside the mobile laboratory.
Temperature data of each individual were modeled with the same set of PCNM (i.e., sinusoidal with exactly the same period), which were constructed using a unique sampling grid for each year, as proposed by Borcard et al. [30].
Lalremruata et al. used a unique sample set from some of the most isolated human communities on the planet, but were limited by the number of loci that they could investigate because so few P. malariae and P. brasilianum samples have been studied genetically.
In this setting, up to 27 different T-cell specificities can be identified in a single cell sample, using a unique two-color code for each pMHC specificity as reported previously (18).
Using a unique data source, newly digitized samples of Egypt's individual-level population censuses of 1848 and 1868, this article documents that the cotton boom in 1861-1865 increased the demand for imported slave labor in Egypt's cotton-favorable districts, where slavery was (almost) non-existent prior to the boom.
Using a unique Internet methodology and a diverse sample of in-school adolescents in Rio de Janeiro, our study addressed gaps and limitations of prior inquiries by examining adolescent knowledge and perceptions of abortion methods outside of clinical settings.
A straightforward method is presented for characterizing the quality of carbon nanotube (CNT) dispersions using scanning electron microscopy (SEM) imaging of samples prepared using a unique procedure, which successfully prevents re-coagulation of the CNTs during the evaporation of the dispersant.
Here we use 454 pyrosequencing to discover microsatellites from a pooled library of post oak samples using reduced representational libraries based on restriction site conservation (Maughan et al., 2009), with each sample labeled independently using a unique multiplex identifier barcode.
Sample extraction was performed using a Unique® ultrasound, model USC 5000A, 40 kHz.
All samples were anonymized by the GP using a unique CPRD identifier code such that no patient identifiable information was included with the sample or questionnaire.
