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From the rest of the culture, plasmids were purified using a typical alkaline lysis procedure and filtration plates (Millipore Multriscreen Filtration System MAGVN2250) to remove protein and cellular debris prior to the plasmid precipitation step.
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In this work, the catalytic activity of as-obtained SW Ni Ni3S2/NF and HC Ni3S2/NF nanostructures towards oxygen evolution reaction (OER) under alkaline media (pH ~13.5) was evaluated using a typical three-electrode system, in which Ni3S2/NF nanostructures were directly used as the working electrode (see electrochemical measurement details).
Electrospinning was performed using a typical setup.
As a typical alkaline earth cations receptor, DBTS also showed spectral changes upon their addition.
In a typical alkaline pretreatment process, the regenerated corn stover was received in a yield of 61.6%.
Element 120 is expected to be a typical alkaline-earth element.
Plasmid DNA was isolated using a standard alkaline lysis protocol.
DNA extractions on individuals from the laboratory colonies were performed using a simple alkaline method [30].
BAC DNA was isolated using a modified alkaline lysis method [23].
GR AF1 activity was determined using a secreted alkaline phosphatase (SEAP) assay as described [7].
Cosmid 7-2-1 was prepared using a standard alkaline lysis procedure.
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