Sentence examples for using a twofold dilution from inspiring English sources
Standard curve was prepared for U6 snRNA using a twofold dilution.
ELISA procedures relevant to the determination of antibody or antigen titers using a twofold dilution series have been described [ 11].
Dose responses were based on a 12-point inhibitor titration, using a twofold dilution series starting from 30 μM to determine the IC50 values of PERK inhibition.
The antibiotic will be titrated into the infected cultures at 4 hours after addition of the chlamydia using a twofold dilution series from 140 g/ml to 0.008 g/ml (in fresh media with cycloheximide 1 g/ml).
In addition, primer efficiency (e) was evaluated on a standard curve generated using a twofold dilution series of a mixed sample over at least five dilution points and verified to be higher than 80% (e = 10^(−10^lope)).
The concentration ranges of the compounds used in the tests (using a twofold dilution system) were 0.0039 0.5 mg/mL for XH and 0.0078 2.0 mg/mL for the extracts.
By using a modified twofold dilution scheme we determined the quantitative culture in CFU/ml.
A twofold dilution series of the 5h (–HU) time point was used to estimate changes in protein levels in the presence of HU.
A twofold dilution of supernatants and 1 μg cell lysate per well were assessed using the SearchLight sEnd assay.
Cultures were diluted to an OD600 of 0.5, and 5 μl of a twofold dilution was spotted on YPD plates containing different concentrations of sorbitol or ethanol.
In parallel, a twofold dilution series of recombinant untagged TFEα/β or TBP was loaded on to the gels to generate a calibration curve used to allow determination of the expression levels.
