Sentence examples for using a triple mutant from inspiring English sources

We analyzed the effect of the combined ablation of p21Cip1 and p27Kip1 in a Cdk4 R24C background using a triple mutant mouse model.

Mathias, J. R., Zhang, Z., Saxena, M. T. & Mumm, J. S. Enhanced cell-specific ablation in zebrafish using a triple mutant of Escherichia coli nitroreductase.

Using a triple mutant of BCR-ABL (Y177F, ΔSH2, and ΔPro), we show that this mutant still confers growth factor independence to 32D cells (Figure 1), but exhibits a profound defect in the transformation of primary hematopoietic cells.

We previously reported, using a triple mutant in the apical loop 1 residues (H83A/R84A/S85A or AAA; Brissett et al., 2007), that this loop is crucially important for selecting the templating base in a 2 nt gap.

To confirm that this was due to the ubiquitylation of Jen1 molecules originating from endosomes, we used a triple ypt6Δ gga1Δ gga2Δ mutant in which retrograde trafficking is abolished.

Additional docking experiments with selective hCYP27B1 or hCYP24A1 inhibitors using both the hCYP27B1 model and a triple mutant hCYP24A1 model provided further support for the importance of H-bonding interactions with the three identified active site amino acids.

A triple mutant of Gap1p, Gap1pK9R,K16R,K76R, which did not contain any ubiquitination site, was used as a negative control.

Meanwhile, a triple mutant K280N/K107H/R102N was also predicted.

Further engineering through random mutagenesis produced a triple mutant M3 with improved thermostability.

In the double mutants and a triple mutant, the single mutations were incorporated in various combinations.

Properties of the single mutants K557R, D591V and K617A, a double mutant, D591V/K617A, and a triple mutant, K557R/D591V/K617A, were evaluated.