Sentence examples for using a triple ligation from inspiring English sources
Plasmids encoding chimeric 'tail-swap' proteins were created using a triple ligation approach and PCR to generate unique sites.
The various PML constructs, ultimately housed in the pcDNA 3.1 vector using a triple ligation strategy, were designed to match the following sequences found in the NCBI data base (PML I: gi/190116/; PML Ib: gi/12275902/; PML IV gi/12275904/; PML VI: gi/67089163/ ).
The nuclear Cre recombinase gene was amplified to include an SV40 large T antigen nuclear localization sequence [26] using the primers listed in Table 2. Cre recombinase and a variable portion of exoS were cloned into pUCP20 in a triple ligation using EcoRI, SacI and SalI restriction sites.
A molecular pairlist was generated using a triple-range cutoff.
The homology arms were ligated in a triple ligation to 4600C-loxP as well as to each other.
In a triple ligation set up, SalI-HpaI restricted Ste2 fragment and HpaI-NotI restricted Fz1 fragment were used to replace the ubc9 insert in pPCUP1-ubc9-CRU using SalI-NotI restriction sites, resulting in pPCUP1-s-Fz1-CRU.
A triple ligation with the pUC-18 GFP plasmid and each half of the ITR was performed with T4 DNA Ligase (Invitrogen) for 1.5 hours at room temperature.
An undocumented NcoI recognition site present in the pYL156 vector backbone sequence necessitated a triple ligation due to the generation of two backbone fragments.
The two fragments were digested and cloned, as a triple ligation, into 5′ -XhoI to 3′ -XbaI cut pJFRC82-20XUAS-IVS-Syn21-GFP-p10 (Pfeiffer et al., 2012).
Barcodes were ligated onto the amplicons using a standard ligation reaction as follows.
