Slides were analyzed blindly on an Olympus BH2 fluorescent microscope, using a triple band-pass filter (Chroma Technology, Brattleboro, VT, USA).
Fluorochrome labelled bone sections were assessed using a triple filter (Zeiss filter set no. 25, Zeiss Axioplan, Carl Zeiss AG) and a fluorescent lamp.
Fluorescent signals in at least 30 nonoverlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA) with a 100× planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colors.
Fluorescent signals in at least 30 non-overlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA, USA) with a × 100 planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colours.
Under an Olympus X51 fluorescent microscope using a triple-filter we determined the percentage of O4-positive cells in relation to >100 DAPI-stained nuclei in randomly selected eye fields for each experimental condition.
A molecular pairlist was generated using a triple-range cutoff.
Images were acquired using a fluorescent microscope with a triple fluorescent filter [DAPI (4′,6-diamidino-2-phenylindole), GFP (green fluorescent protein) and Texas Red].
Enumeration of HER-2/ neu and CEP 17 probe signals was performed under fluorescence microscopy with a 100-watt mercury lamp at 1000X magnification using a Zeiss Axiophot fluorescent microscope with triple bands filters.
Fluorescent in situ hybridization was performed according to the manufacturer's protocol using a red fluorescent probe specific for mouse chromosome 5 and a green fluorescent probe for chromosome 7 (Applied Spectral Imaging).
