Under an Olympus X51 fluorescent microscope using a triple-filter we determined the percentage of O4-positive cells in relation to >100 DAPI-stained nuclei in randomly selected eye fields for each experimental condition.
Slides were analyzed blindly on an Olympus BH2 fluorescent microscope, using a triple band-pass filter (Chroma Technology, Brattleboro, VT, USA).
Fluorescent signals in at least 30 nonoverlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA) with a 100× planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colors.
Fluorescent signals in at least 30 non-overlapping interphase nuclei with intact morphology were scored using a Zeiss Axioplan 2 microscope (Carl Zeiss Meditec, Inc., Dublin, CA, USA) with a × 100 planar objective, using a triple band-pass filter that permits simultaneous blue, green, and red colours.
Using a triple-band bypass filter (Chroma Technology Corp. set: 61000 v2SBX for DAPI/FITC/TRITC) the interaction of TRITC/Cytokeratin (ex.542 nm em.560 nm), FITC/uPA, MMP-1, MMP-7 (ex.488 nm em.505 530 nm) and DAPI (ex.360 em.460) were examined.
Cells expressing mCherry-tagged proteins were visualized using a Pinkel triple filter set (Semrock, Rochester, NY) and a TIRF 100× 1.49 NA oil-immersion lens.
Simple visual inspection using a CFP/YFP/RFP triple filter cube plainly revealed a wide variety of maturation stages (Figure 7A).
A molecular pairlist was generated using a triple-range cutoff.
Data acquisition was performed using a Zeiss Axioplan 2 fluorescent microscope equipped with a triple filter, Fluoarc fluorescent light source and Axiocam ICc1 camera.
The specimens were counterstained with 4,6-dianidino-2-phenylindone (DAPI) and examined using a PM-2000 imaging system (HistoRx, New Haven, CT, USA) equipped with a triple filter set (Chroma, Bellows Falls, VT, USA): no.
