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Proteins were separated using a tricine based gel system (as above).
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The chromatographic results were also corroborated using a Tricine SDS-PAGE analysis.
Protein extraction was carried out as previously described (Schoenman et al., 2010; Campion et al., 2012), and running samples were prepared from muscle extracts semi-purified for PV using tris-tricine based buffer system.
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We describe a microfluidic Western blot assay (μWestern) using a Tris tricine discontinuous buffer system suitable for analyses of a wide molecular mass range (6.5 116 kDa).
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For separation of low molecular weight polypeptides, SDS-PAGE was carried out using a Tris-Tricine buffer system according to the protocol of Schagger and von Jagow [ 22].
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