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Cell migration was detected using a transwell plate (Corning, Lowell, MA) followed by DAPI staining.
Synoviocyte and cartilage were co-cultured using a transwell plate with a 0.4 μm microporous insert (Corning Inc., Corning, NY).
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Additionally, we confirmed the effects on cell migration using a transwell assay, in which MIAMI cells expressing lamin A or progerin were plated in the upper chamber of a transwell system in MIAMI media containing no FBS.
Using a transwell system, all chondrosarcoma cell lines showed migratory properties, and started migrating ∼30 min after plating, except for JJ012 cells, which started migrating only 4 h after plating (results not shown).
The migration of cells was examined using a Transwell chamber.
We then assessed EPC migration using a transwell assay.
Cell migration was measured using a transwell chamber.
EPCs migration was evaluated using a transwell migration assay.
Cell migration was measured using a Transwell chamber (Millipore, Germany).
These results were confirmed using a transwell chamber assay.
Cell invasion was assessed using a transwell matrigel invasion assay.
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