The migration assay was performed using a transwell chamber, consisting of 8 mm membrane filter inserts (Corning, New York, USA) coated with Matrigel (BD Biosciences, California, USA).
To test whether cancer cells instruct surrounding fibroblasts to secrete factors such as HGF to promote tumor growth, we co-cultured NAF #200N with MDA-MB-468 cells using a transwell insert containing a 0.4-µm polyester membrane.
The invasion and motility assays were performed using a Transwell chamber with a 8-μm pore size polycarbonate membrane (Corning, Hangzhou, China) as recommended by the supplier.
A transwell invasion assay was performed using a transwell system (24 wells, 8 μm pore size with poly-carbonate membrane) and Matrigel according to the manufacturer's instructions.
A cell migration assay was performed using a transwell system, which allows cells to migrate throughout an 8- μm pore size polycarbonate membrane of millicell.
Tumor cell migration was analyzed using a transwell chamber (Corning Coster, Corning, NY, USA) provided with an 8 μm pore polycarbonate membrane.
Using a transwell system, we functionally demonstrated that fewer cells in the ME1-repressed group migrated or invaded into the lower surface of the transwell membrane.
Cell invasion was measured using a Transwell system (Corning, Rochester, NY, USA) that allows cells migrate through 8-μm pores in polycarbonate membranes.
The same experiment was also performed using a transwell co-culture system in which Hospicells were cultured in the bottom chamber and OVCAR3 cells were cultured in the upper membrane.
The migration of cells was examined using a Transwell chamber.
