LoVo cell invasion was detected using a transwell chamber and Matrigel.
Invasive ability of cells was tested using a transwell insert pre-loaded with Matrigel (BD Biosciences, San Diego, CA).
Cell migration and invasion were gauged using a Transwell migration assay and a Matrigel invasion assay (8 μm pore size; BD Falcon, San Jose, CA, USA) as previously described.
As shown in Figure 1c, compared to TSP50-expressing CHO and MCF-10 cells, only a few control cells were capable of penetrating the Matrigel layer using a transwell chamber assay.
Next, the ability to invade Matrigel was assessed using a Transwell assay.
Invasion of cells through matrigel was determined using a Transwell system (CHEMICON) as described previously [ 28]. α-thrombin was added at 15 nM, TFLLR-NH2 was added at 30 μM and SCH79797 was added at 35, 70, or 150 nM to the cells (0.5×10cells/well) in the upper well containing serum-free medium.
Experiments were performed using a Transwell filter system in the presence or absence of Matrigel as well as using organotypic brain slices (Fig. 4).
A transwell invasion assay was performed using a transwell system (24 wells, 8 μm pore size with poly-carbonate membrane) and Matrigel according to the manufacturer's instructions.
The migration assay was performed using a transwell chamber, consisting of 8 mm membrane filter inserts (Corning, New York, USA) coated with Matrigel (BD Biosciences, California, USA).
The migration of cells was examined using a Transwell chamber.
