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To test whether cancer cells instruct surrounding fibroblasts to secrete factors such as HGF to promote tumor growth, we co-cultured NAF #200N with MDA-MB-468 cells using a transwell insert containing a 0.4-µm polyester membrane.
Tumor cell migration and invasion were carried out using a Transwell insert (8 μm, Corning, NY).
Resistance measured by using a transwell insert with media and no endothelial cells was considered a blank reading.
Invasive ability of cells was tested using a transwell insert pre-loaded with Matrigel (BD Biosciences, San Diego, CA).
To this aim, MSCs seeded in 3D scaffolds or 2D surfaces were co-cultured with macrophages using a transwell insert system and levels of soluble factors related with inflammation and chemotaxis were quantified.
Activated γδ T cells and activated CD4+ T cells were co-cultured with osteoclast precursor cells to permit cell contact, or cells were separated using a Transwell insert into which the activated T cells were placed.
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For coculture experiments, we used a Transwell insert with 1- μm pores, whereby AG1522 cells destined to be bystanders were grown to the confluent state at the bottom side of the inserts.
We used a Transwell insert (24-well insert, pore size 8 μm; Corning, Inc., Corning, NY) to determine the effect of miR-339-5p miR-339-5p miR-339-5p migration and invasion in vitro.
Synoviocyte and cartilage were co-cultured using a transwell plate with a 0.4 μm microporous insert (Corning Inc., Corning, NY).
The migration assay was performed using a transwell chamber, consisting of 8 mm membrane filter inserts (Corning, New York, USA) coated with Matrigel (BD Biosciences, California, USA).
The migration of cells was examined using a Transwell chamber.
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