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Cell migration assays were carried out using a transwell filter migration assay as previously described [ 12].
Cell migration assays were carried out using a transwell filter migration assay as previously described (Qualtrough et al, 2007).
Experiments were performed using a Transwell filter system in the presence or absence of Matrigel as well as using organotypic brain slices (Fig. 4).
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The migration assay was performed using a transwell chamber, consisting of 8 mm membrane filter inserts (Corning, New York, USA) coated with Matrigel (BD Biosciences, California, USA).
The chemotactic migration of ECs was assayed using a Transwell chamber with 6.5 mm diameter polycarbonate filters (8 μm pore size).
The migration of cells was examined using a Transwell chamber.
We then assessed EPC migration using a transwell assay.
Cell migration was measured using a transwell chamber.
EPCs migration was evaluated using a transwell migration assay.
Cell invasion was assessed using a transwell matrigel invasion assay.
Cell migration was measured using a Transwell chamber (Millipore, Germany).
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