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The migration of cells was examined using a Transwell chamber.
Cell migration was measured using a transwell chamber.
Migration activity of hNSCs was tested using a Transwell chamber system.
The migration assay was carried out using a transwell chamber with an 8-µm pore size (Costar #3422, Corning, NY).
As expected in untransfected SH-SY5Y cells, our migration assay using a transwell chamber showed a positive NRG1-stimulated migration in control-vector transfected cells (Figure 8).
Cell migration was measured using a Transwell chamber (Millipore, Germany).
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The invasion and migration activity of 5-8F, 6-10B and siNM23-H1-transfected 5-8F cells was assayed using a transwell cell culture chamber as described before (Albini et al, 1987).
To assess the effect of FOXP1 on neuroblastoma cell motility, two different assays were used: a transwell (Boyden chamber) assay and a wound healing assay.
Migration assays were performed using a modified Transwell chamber (Corning, Inc). in a 24-well plate format and cell culture inserts containing filters with a pore size of either 3 μm (for neutrophilic granulocytes) or 5 μm (for PBMCs).
The migration assay of cells was performed with a Transwell chamber using a 24-well plate and polycarbonate filters (Transwell Costar, Corning, Acton, MA) with a pore size of 8 μm as previously described [ 25]. 10 McellsE1 cells in 50 μl DMEM were seeded in the upper compartment.
Exogenous VEGF at 25 ng ml−1 did not increase the migration of A549 cells through a collagen-coated membrane after 4 h of incubation using a 24-transwell chamber assay.
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