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Using a transcript of Gagarin's original mission communications, I began to story-board the film, listing the different camera angles we'd need from the cupola to illustrate what he'd seen.
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Using a transcript count of 32 as a lower bound, we were able to define a threshold for miRNA expression based on transcript abundance.
The combination of reads derived from a single set of transcriptomic data (30 million reads) enables comprehensive examination of ∼3000 of the gene models in the Bestmodelv1 set using a transcript sequence coverage depth of 23.
We used a transcript profiling approach to identify targets of estrogen and c-Myc in breast cancer cells.
Transcriptomes of various germinating tissues in barley have been determined using a variety of transcript profiling technologies [ 31- 35].
Silk-preferential genes were selected using a criterion of transcript abundances in silk twice greater than in all the other tissues.
Variants can be input in a number of formats (VCF, Human Genome Variation Society HGVSS) and so on) and the functional consequence annotated using a number of transcript sets (Ensembl, Gencode or Refseq).
Remaining reads were independently assembled de novo three times using Trinity with default parameters, using a lower transcript size cut-off of 200 nt and jaccard-clipping, but including a minimum kmer coverage of 2, 4 and 10 (−−min_kmer_cov parameter).
This approach identified transcripts from 4699 genes as present in at least one copy in 100,000 reads; using an average transcript size of 1.3 kb, this means the low detection cut-off was approximately equivalent to an average RPKM of 7.7.
Using a corpus compiled of transcripts of the sitcom The Big Bang Theory, instances containing conventionalised forms of request are first extracted and then manually examined to make sure that all the remaining instances express requests and thus qualify for further analysis.
Starting with human liver RNA, I was neither able to amplify any threonine aldolase transcripts using a variety of 5' and 3' RACE methods nor to detect any transcripts in a wide range of tissue and cell line cDNAs by RT-PCR.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com