The authors were able to radiolabel the 3-formyl analogue 18 in tert-butanol in 5 min at room temperature using a total volume of 100 μL.
The CPB circuit was primed using a total volume of 21.1 mL priming solution composed of 10 mL of Plasma-Lyte A (Baxter, Deerfield, IL, USA), 10 mL of 6%% Hetastarch (Hospira, Lake Forest, IL, USA), 0.8 mL of 0.406 mEq/mL magnesium sulfate (Magnesium Sulfate, APP Pharmaceuticals, Schaumburg, IL, USA), and 0.3 mL of 3.3 mmol/mL THAM Solution (XVIVO Perfusion AB, Göteborg, Sweden).
HIV-1 exposures were performed essentially as previously described using a total volume of 2 10 µL (rectal: 170 ng p24) or 200 µL (intravenous: 58 ng p24) [32], [32].
Small subunit rDNA sequences were PCR-amplified using a total volume of 25 µl containing 1 2 µl of primer, 1 5 µl of DNA template, 18 23 µl of water and puReTaq Ready-to-go PCR beads (GE Healthcare, Quebec, Canada).
qPCR reactions were performed in a 384-well plate using a total volume of 10 μl/well.
OPT underwent hydrothermal treatment using a total volume of 2-L-scale 2-L-scale 2-L-scaler (Parr Instirredts, Moline, IL, USA).
We used a total volume ranging from 20 to 30 ml, the actual volume given depending on joint capsule distensibility before rupture.
The remainder of the protocol was carried out using the instructions provided with the RNeasy Mini kit (Qiagen), with the exception of the elution step, which used a total volume of 40 μL RNase-free water (Sigma).
Polymerase chain reaction (PCR) was performed in a thermal cycler (Applied Biosystems® Veriti®, California, USA) using a total reaction volume of 10 μl.
following the manufacturer's guidelines in 24- to 34-plex reactions in 384-well plates using a total reaction volume of 5 µl including 12.5 ng of genomic DNA.
Total amount of radioactivity in the blood was calculated using a total blood volume based on gender and individual body weight and height and expressed as % injected dose of I-MIBG [ 15– 17].
